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The sea urchin Strongylocentrotus nudus is highly sensitive to oil pollution. Experiments were performed in winter, spring and summer over periods of 15 to 45 days. Thermal stimulation by Evdokimov's method was applied to obtain mature sexual products during winter and spring tests. The gonads were studied histologically and morphometrically, and the sexual cells obtained were analyzed at the embryological level. No histological and morphometrical differences were recorded between sexual cells of controls and experimentals.
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Since these vegetable oils contain bioactive compounds, it is important for toxicology studies to characterize the toxicities of the dosing vehicles themselves. It has been recently proposed that the width of the genital tubercle GT , the dorsal—ventral length D—V length of the GT, and urethral tube closure in mouse fetuses can be used as novel markers for monitoring sexual development in mice. However, how these parameters are influenced by the dosing vehicles themselves remains unclear. Our results showed that all three vehicles influenced GT width and D—V length, but not GT morphology, suggesting that the effects of dosing vehicles themselves might need to be considered when GT width or D—V length is used as a parameter to evaluate the effects of chemicals on GT development. Since the control groups in such studies are treated with the vehicle only, the effects of the vehicle itself on animals should be clarified.
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  • added: 08.06.2019
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