Journal of Animal Science and Biotechnology. December , Cite as. Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis 2-DE results using two commercial antibodies. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation, and the guanylate cyclase pathway. The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation.
Conners is a Board Certified urologist and fellowship trained surgeon who has trained students, residents, and fellows through Harvard Medical School over the last decade. He is nationally recognized as a top male reproductive and infertility specialist in the Boston area. There are many causes of male infertility: sperm blockage, poor sperm quality, low sperm counts, hormones, lifestyle choices, and medications, can all play a part in male reproduction concerns.
At New England Cryogenic Center, we understand the priceless quality of the materials we store and ship. From sperm to embryos, and from stem cells to bone marrow, the materials entrusted to us are invaluable. Consequently, we perform our jobs with incredible care. We have enormous pride in our role in helping people achieve their dreams of parenthood.